Clinical Pharmacist and Master’s student in Clinical Pharmacy with research interests in pharmacovigilance, behavioral interventions in mental health, and AI applications in clinical decision support. Experience includes digital health research with Bloomsbury Health (London) and pharmacovigilance practice in patient support programs. Published work covers drug awareness among healthcare providers, postpartum depression management, and patient safety reporting. 
Clinical Pharmacist and Master’s student in Clinical Pharmacy with research interests in pharmacovigilance, behavioral interventions in mental health, and AI applications in clinical decision support. Experience includes digital health research with Bloomsbury Health (London) and pharmacovigilance practice in patient support programs. Published work covers drug awareness among healthcare providers, postpartum depression management, and patient safety reporting. What was studied?
This original research article investigated how a high-fat diet (HFD) affects heavy metal accumulation and toxicity in mice, with particular focus on the role of gut microbiota in these processes. The study compared two groups of mice: one fed a normal diet (ND) and another fed an HFD, both exposed to arsenic (As), cadmium (Cd), or lead (Pb) at 100 ppm in drinking water for ten weeks. The key objective was to elucidate the relationship between dietary fat content, gut microbiota composition, and the body’s ability to detoxify and excrete heavy metals, which is central to heavy metal certification programs. The researchers systematically measured heavy metal accumulation in the liver and kidney, excretion through feces, and assessed organ function damage using biomarkers. They also used 16S rRNA sequencing to analyze gut microbiota responses to heavy metal exposure under different dietary conditions, aiming to identify specific microbial genera associated with heavy metal detoxification.
Who was studied?
The study utilized eight-week-old male C57BL/6 mice, a commonly used laboratory strain, obtained from the Guangdong Medical Laboratory Animal Center in China. The mice were randomly assigned to eight groups with six mice per group, ensuring both ND and HFD groups and controls for each of the three heavy metals (As, Cd, Pb). All animals were maintained in a controlled environment with free access to food and water, and were exposed to heavy metals via drinking water for a continuous period of ten weeks. At the end of the experiment, samples were collected for biochemical and microbiological analyses. This controlled animal model allowed for precise examination of diet-specific effects on both gut microbiota and heavy metal metabolism, with relevance for extrapolation to human health and regulatory frameworks.
Most important findings
| Finding Category | Key Results Detail |
|---|---|
| Heavy Metal Accumulation | HFD-fed mice accumulated significantly more As, Cd, and Pb in the liver and kidney compared to ND-fed mice after exposure, indicating impaired detoxification and a higher risk. |
| Organ Function Damage | Biomarkers of liver (ALT, AST) and kidney (TPU, UCrea, UUA) damage were elevated in HFD-fed mice exposed to heavy metals, signifying greater toxicity. |
| Fecal Heavy Metal Excretion | HFD-fed mice excreted less As, Cd, and Pb through feces than ND-fed mice, implicating reduced elimination via the gut pathway. |
| Gut Microbiota Composition | HFD and ND mice exhibited distinctly different gut microbiota profiles; HFD-fed mice showed greater loss in microbial abundance/diversity upon heavy metal exposure. |
| Microbial Genera Response | In ND-fed mice, certain genera (Bacteroides, Lactobacillus, Butyricimonas, Dorea, Coprococcus, Desulfovibrio, Roseburia, Prevotella) increased in response to metals. |
| Correlation with Detoxification | Positive correlation between fecal metal content and abundance of total gut microbiota and specific genera suggests these microbes aid in excretion/detoxification. |
| Implications for Probiotics | Lactobacillus and other genera enriched in ND-fed, metal-exposed mice correlated with reduced organ metal accumulation, indicating potential for probiotic intervention. |
Key implications
This study demonstrates that high-fat diets can compromise the gut microbiota’s natural ability to detoxify heavy metals, leading to greater organ accumulation and toxicity. For heavy metal certification programs, it underlines the necessity of considering dietary patterns and gut microbiota composition in risk assessment and mitigation strategies, and supports the development of microbiota-targeted interventions, such as probiotics, to enhance heavy metal detoxification.
Citation
Liu T, Liang X, Lei C, Huang Q, Song W, Fang R, Li C, Li X, Mo H, Sun N, Lv H, Liu Z. High-Fat Diet Affects Heavy Metal Accumulation and Toxicity to Mice Liver and Kidney Probably via Gut Microbiota. Front Microbiol. 2020;11:1604. doi:10.3389/fmicb.2020.01604
Heavy metals are high-density elements that accumulate in the body and environment, disrupting biological processes. Lead, cadmium, arsenic, mercury, nickel, tin, aluminum, and chromium are of greatest concern due to persistence, bioaccumulation, and health risks, making them central to the HMTC program’s safety standards.
Cadmium is a persistent heavy metal that accumulates in kidneys and bones. Dietary sources include cereals, cocoa, shellfish and vegetables, while smokers and industrial workers receive higher exposures. Studies link cadmium to kidney dysfunction, bone fractures and cancer.
Lead is a neurotoxic heavy metal with no safe exposure level. It contaminates food, consumer goods and drinking water, causing cognitive deficits, birth defects and cardiovascular disease. HMTC’s rigorous lead testing applies ALARA principles to protect infants and consumers and to prepare brands for tightening regulations.
